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  "source": "independently verified native state; original descriptions retain imported attribution",
  "id": "P-OPP-01448",
  "title": "Create self-fertilising rice that feeds its own soil microbes",
  "done_when": "",
  "criteria_revision": null,
  "summary": "Independent review by claude-independent-prior-art-reviewer-20261106: No self-fertilising rice exists in farmers' fields, so the Main KPI of 150,000,000 hectares is unmet. The closest result is UC Davis rice edited by CRISPR to accumulate and exude apigenin, which promoted biofilm formation by soil nitrogen-fixing bacteria and raised grain yield under limited soil nitrogen in limited experiments (Yan et al., Plant Biotechnol J 2022, https://escholarship.org/uc/item/3xs0w2f2); a November 2025 follow-up identified 20 plant compounds for wheat (https://sciencedaily.com/releases/2025/11/251123115435.htm). Engineering nitrogenase into rice has reached expression of NifH and NifB components, not fixation; oxygen sensitivity remains the core obstacle. Legume-style nodulation in cereals is still basic science: 2026 Science and Science Advances papers on NodD signalling and the NIN regulator produced nodule-like structures that could not complete the symbiosis (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013914/). Pivot Bio sells nitrogen-fixing microbes for maize, sorghum, wheat and cotton, but rice is not on its 2026 list and its yield claims are company data. Honest limit: microbial inoculants and the apigenin rice concept are published, so tasks that only re-run them are known answers; the open work is rice that measurably supplies its own nitrogen at field scale, and each task needs its own basis. (open solution found: no; known answer: no; 6 project-level searches of api.github.com/search/repositories, export.arxiv.org/api/query, api.crossref.org/works; draft af27a944228f98a9a083803f73e7cb873e6930edb80eccbfddc002b81d3c29e1, source 91492d199fe40a76d945cccb9d108535271454a9f50bf10baf6f2018bac4bdc1)",
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  "prior_art": {
    "basis": "Independent review by claude-independent-prior-art-reviewer-20261106: No self-fertilising rice exists in farmers' fields, so the Main KPI of 150,000,000 hectares is unmet. The closest result is UC Davis rice edited by CRISPR to accumulate and exude apigenin, which promoted biofilm formation by soil nitrogen-fixing bacteria and raised grain yield under limited soil nitrogen in limited experiments (Yan et al., Plant Biotechnol J 2022, https://escholarship.org/uc/item/3xs0w2f2); a November 2025 follow-up identified 20 plant compounds for wheat (https://sciencedaily.com/releases/2025/11/251123115435.htm). Engineering nitrogenase into rice has reached expression of NifH and NifB components, not fixation; oxygen sensitivity remains the core obstacle. Legume-style nodulation in cereals is still basic science: 2026 Science and Science Advances papers on NodD signalling and the NIN regulator produced nodule-like structures that could not complete the symbiosis (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013914/). Pivot Bio sells nitrogen-fixing microbes for maize, sorghum, wheat and cotton, but rice is not on its 2026 list and its yield claims are company data. Honest limit: microbial inoculants and the apigenin rice concept are published, so tasks that only re-run them are known answers; the open work is rice that measurably supplies its own nitrogen at field scale, and each task needs its own basis. (open solution found: no; known answer: no; 6 project-level searches of api.github.com/search/repositories, export.arxiv.org/api/query, api.crossref.org/works; draft af27a944228f98a9a083803f73e7cb873e6930edb80eccbfddc002b81d3c29e1, source 91492d199fe40a76d945cccb9d108535271454a9f50bf10baf6f2018bac4bdc1)",
    "searches": [
      "api.github.com/search/repositories \"self-fertilising rice feeds soil microbes\" url=https://api.github.com/search/repositories?q=self-fertilising%20rice%20feeds%20soil%20microbes&per_page=10 sha256=4af480b8ee5b87b369a76c49bd22c9a783908272ebffbe97898f8ab0f0772a5f",
      "export.arxiv.org/api/query \"self-fertilising rice feeds soil microbes\" url=https://export.arxiv.org/api/query?search_query=all%3Aself-fertilising%20AND%20all%3Arice%20AND%20all%3Afeeds%20AND%20all%3Asoil%20AND%20all%3Amicrobes&start=0&max_results=10 sha256=6107a25e8ed40691021be5c102e5f1b3cfafcb54ea51875d5244891cedebde23",
      "api.crossref.org/works \"self-fertilising rice feeds soil microbes\" url=https://api.crossref.org/works?query=self-fertilising%20rice%20feeds%20soil%20microbes&rows=10&select=DOI,title sha256=708c2148ea4cf49c632cacb5f9d354f7bdbbd582c431271e069e4b60acb1dccb",
      "api.github.com/search/repositories \"self-fertilising rice feeds agriculture land crop\" url=https://api.github.com/search/repositories?q=self-fertilising%20rice%20feeds%20agriculture%20land%20crop&per_page=10 sha256=4af480b8ee5b87b369a76c49bd22c9a783908272ebffbe97898f8ab0f0772a5f",
      "export.arxiv.org/api/query \"self-fertilising rice feeds agriculture land crop\" url=https://export.arxiv.org/api/query?search_query=all%3Aself-fertilising%20AND%20all%3Arice%20AND%20all%3Afeeds%20AND%20all%3Aagriculture%20AND%20all%3Aland%20AND%20all%3Acrop&start=0&max_results=10 sha256=4e5ca5dc5a2970e61c341333367e5a1718bd768440105274e4d3713b011e1d56",
      "api.crossref.org/works \"self-fertilising rice feeds agriculture land crop\" url=https://api.crossref.org/works?query=self-fertilising%20rice%20feeds%20agriculture%20land%20crop&rows=10&select=DOI,title sha256=07036fd8e035f918d4760a4491e74f3be9e22ddc8e903d207192bc8f5659895b"
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